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KMID : 0988420030150010026
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Journal of Dental Science (C.N.U.)
2003 Volume.15 No. 1 p.26 ~ p.41
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The Effects of Ginseng Saponins on the Periodontal Rearrangement of Experimentally Moved Molar in the Rat
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Abstract
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This study was performed to analyze the effects of ginseng total saponins on the activity of human periodontal ligament cells in vitro and on the in vivo periodontal rearrangement of experimentally moved molar in the rat.
For in vitro experiment, the periodontal ligament cells were isolated from extracted sound tooth of patient who was taken the orthodontic treatment. Ginseng total saponins of 0, 1, 10, 100, 500 ㎍/㎖ was administered into each cultured cells. The cell proliferation and activity were examined through measurement of absorbance and RT-PCR analyses. For in vivo experiment, the latex elastic band was inserted between the first and second maxillary molars on right and left sides of rats for 3 weeks. From 3 days before the removal of elastic latex band, they were injected intraperitoneally with ginseng total saponins of 50, 100, 200 mg/kg in each experimental group and saline solution in control group everyday. And they were sacrificed according to the 5-day periods from the day of removal of elastic latex bands and were compared by histological examination among the groups.
The results were obtained as follows.
1. There was no difference of the absorbance in human periodontal ligament cells between the experimental of 0, 1, 10, 100 ㎍/㎖ ginseng saponins and control groups, while it was decreased in the group of 500 ㎍/㎖ ginseng saponins.
2. The gene expression of ALP, fibronectin, DSP3, and COL1 was increased in the group of 1, 10 ㎍/㎖ ginseng saponins, while it was decreased greatly except COL1 in the group of 100 ㎍/㎖.
3. There was no difference of the gene expression of TGF-β_(1) between the experimental of 1, 10 ㎍/㎖ and control groups, while it was decreased greatly in the group of 100 ㎍/㎖.
4. The rearrangement of collagen fibers in the periodontal ligament of rats appeared faster in the experimental groups than in the control group.
5. The remodelling of alveolar bone of rats appeared faster in the experimental groups than in the control group.
These results indicate that ginseng total saponins from red ginseng increased the activity of periodontal ligament cells and suggest that ginseng total saponins may stimulate the periodontal rearrangement after orthodontic treatment. So retention period after tooth movement can be decreased.
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